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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Cell Mol Med. 2010 Apr 19;15(5):1087–1095. doi: 10.1111/j.1582-4934.2010.01072.x

Fig. 7.

Fig. 7

CCN2 promotes mHSC cell adhesion to FN. Day 3 primary mHSC were pre-incubated with or without 10 μg/ml anti-integrin α5β1 for 30 min and then placed for 30 mins in 96-well tissue culture plates (105cells/well in 100 μl of medium) pre-coated with FN (2 μg/ml) in the presence or absence of 200 ng/ml CCN21-4 or CCN24. Wells were washed and the frequency of adherent cells was evaluated using a CyQuant NF Assay Kit. Data are representative of 3 separate experiments. Values represent mean ±SD of quadruplicate determinations from 3 experiments. *p<0.05 vs Ctrl group; #p<0.05 vs IgG group.