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. Author manuscript; available in PMC: 2010 Oct 21.
Published in final edited form as: J Neurosci. 2010 Apr 21;30(16):5713–5723. doi: 10.1523/JNEUROSCI.1001-10.2010

Figure 6. Retrograde tracing from CeA labels VTA LepRb neurons.

Figure 6

The retrograde tracer fluorogold (FG) was stereotaxically injected into the CeA of LepRbEGFP animals to determine the potential projection of VTA LeRb neurons to the CeA by colocalization of FG and EGFP-IR. (A) Schematic diagram and (B) fluorescent image (FG, red; EGFP, green) of CeA injection site in representative animal. (C,D) demonstrate distribution of FG- and EGFP-IR neurons at two different levels of the VTA. Images below are digital zooms of the boxed areas showing (left to right), merged images, FG-IR, and EGFP-IR. Arrows indicate colocalized neurons. Red star indicates the basolateral amygdala. Scale bars are 200 μm in B and C and 25 μm in inset panels.