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. Author manuscript; available in PMC: 2010 Oct 21.
Published in final edited form as: J Neurosci. 2010 Apr 21;30(16):5713–5723. doi: 10.1523/JNEUROSCI.1001-10.2010

Figure 7. Retrograde tracing from IPAC labels VTA LepRb neurons.

Figure 7

The retrograde tracer fluorogold (FG) was stereotaxically injected into the IPAC of LepRbEGFP animals to determine the potential projection of VTA LeRb neurons to the IPAC by colocalization of FG and EGFP-IR. (A) Schematic diagram and (B) fluorescent image (FG, red; EGFP, green) of IPAC injection site in representative animal. (C) demonstrates distribution of FG- and EGFP-IR neurons in the VTA. Images below are digital zooms of the boxed areas showing (top to bottom), merged images, FG-IR, and EGFP-IR. Arrows indicate colocalized neurons. Red star indicates the basolateral amygdala. Scale bars are 200 μm.in B and C and 20 μm in inset panels.