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Blockade of trk receptors does not rescue expression of non-cholinergic transmitters. 10 nM K252a were applied from 5–24 hr. No significant difference in expression of GABA, glycine or glutamate was observed between the blank and muscle sides of the dish (n>5 cultures per condition per transmitter, >100 neurons per culture). Higher concentrations (up to 10 μM) were also ineffective. Error bars indicate SEM. The Kruskal-Wallis test was used to determine statistical significance.
