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. Author manuscript; available in PMC: 2010 May 6.
Published in final edited form as: Microbiology (Reading). 2009 May 7;155(Pt 8):2664–2675. doi: 10.1099/mic.0.027714-0

Fig. 1.

Fig. 1

Allelic exchange of the accD6 locus of M. smegmatis. (a–c) Genetic organization, partial restriction map and expected hybridization profiles of the accD6 chromosomal region in (a) the wild-type strain mc2155, (b) the single crossover strain D6SCO1 and (c) the M. smegmatis D6DCO2 conditional mutant. Oligonucleotides used to confirm single and double crossover events by PCR are indicated. (d) Southern blot analysis of M. smegmatis accD6 mutants. Three mutants were picked at random (1, 2 and 3); chromosomal DNA was digested with EcoRV and probed for hybridization with a labelled 666 bp fragment corresponding to the 5′ region of kasB. M. smegmatis mc2155 DNA was included as a control (wt). Molecular masses are indicated in kb.