Table 1.

Bacterial strains and plasmids used in this work

Plasmids	Relevant genotype and/or information*	Source or reference
pET28a(+)	Phagemid vector (Kmr lacZ′) for expression of recombinant proteins under control of strong T7 transcription and translation signals	Novagen
pD6MT	pET28a(+) with M. tuberculosis accD6 His-tag fusion gene, under T7 promoter control, Kmr	This study
pD6MS	pET28a(+) with M. smegmatis accD6 His-tag fusion gene, under T7 promoter control, Kmr	This study
pD5	pET28a(+) with M. tuberculosis accD5 His-tag fusion gene, under T7 promoter control, Kmr	Gago et al. (2006)
pA3	pET28a(+) with M. tuberculosis accA3 His-tag fusion gene, under T7 promoter control, Kmr	Gago et al. (2006)
pUC4K	Contains aphA-3 cassette that confers Kmr	Vieira & Messing (1982)
pPR27	E. coli–Mycobacterium shuttle vector, oriM temps, sacB xylE Gmr	Pelicic et al. (1997)
pPR27D6	pPR27 derivative carrying accD6::aphA-3, Gmr Kmr	This study
pCG76	E. coli/Mycobacterium shuttle vector, oriM temps, Str/Spr	Guilhot et al. (1994)
pCGD6	pCG76 derivative harbouring accD6 plus 500 bp, both upstream and downstream of this ORF from M. smegmatis, Str/Spr	This study
pCGHD6	pCG76 derivative harbouring M. smegmatis accD6 His-tag fusion gene, under GroEL promoter control, Str/Spr	This study
E. coli
DH5α	E. coli K-12 F− ΔlacU169 (φ80lacZΔM15) endA1 recA1 hsdR17 deoR supE44 thi-1 λ− gyrA96 relA1	Hanahan (1983)
BL21λ(DE3)	E. coli B F− ompT ${\text{r}}_{\text{B}}^{-}{\text{m}}_{\text{B}}^{-}$ (DE3)	Studier & Moffatt (1986)
BL21λ(DE3) Codon Plus	E. coli B F− ompT hsdS ( ${\text{r}}_{\text{B}}^{-}{\text{m}}_{\text{B}}^{-}$)dcm+ TetR gal (DE3) endA Hte [argU ileY leuW], CmR	Stratagene
M. smegmatis
mc2155	Fast-growing strain harbouring all plasmids used herein	Snapper et al. (1990)
D6SCO1	mc2155 with pPR27D6 integrated into accD6 locus, Kmr	This study
D6DCO2	Mutant containing an intrachromosomal allelic exchange at accD6 locus in presence of pCGD6, Kmr Str/Spr	This study
MS-CG76	mc2155 harbouring pCG76, Str/Spr	This study
MS-CGD6	mc2155 harbouring pCGD6, Str/Spr	This study
MS-CGHD6	mc2155 harbouring pCGHD6, Str/Spr	This study

^*Gm^r, Gentamicin resistance; Km^r, kanamycin resistance; St^r, streptomycin resistance; Sp^r, spectinomycin resistance; Cm^r, cloramphenicol resistance; temp^s, temperature sensitivity.