1×105 c.f.u. were used to inoculate Middlebrook 7H9 supplemented with ADS (10 % v/v) in a 96-well plate containing twofold dilutions of the tested compound. Plates were sealed and incubated at 37 °C for 2 days (M. smegmatis and M. fortuitum), 5 days (M. kansasii) or 7 days (M. tuberculosis and M. bovis BCG). Viability was estimated by adding resazurin followed by further incubation for 24 h. Colour change from blue to pink indicated viability. MIC was determined as the lowest concentration of drug giving no colour change.