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. Author manuscript; available in PMC: 2010 Oct 28.
Published in final edited form as: J Neurosci. 2010 Apr 28;30(17):5843–5854. doi: 10.1523/JNEUROSCI.0137-10.2010

Figure 2.

Figure 2

Fibrinogen regulates neurocan expression in primary astrocytes. A, Increased expression of neurocan mRNA in primary astrocytes after fibrinogen treatment as determined by quantitative PCR and normalized to GAPDH. Results are from three independent experiments performed in duplicate. B and C, Potent and rapid fibrinogen-induced neurocan protein secretion by primary astrocytes with TGF-β (B) or EGF (C) as positive controls. D, Fibrinogen-induced neurocan expression is concentration-dependent. Primary astrocytes were treated for 2 days with fibrinogen (0.1–2.5 mg/ml). TGF-β served as a positive control. Representative blots are shown from three independent experiments. Values are mean ± SEM of three independent experiments. *p < 0.01.