Figure 9.

EIG121 knockdown decreases cell viability under conditions of serum deprivation or cytotoxic drug treatments. (a) MCF-7 cells were transfected with control nontargeting siRNA or EIG121 siRNA for 48 h and incubated in medium with 10% FBS or serum-free medium for 48 h or treated with 20 nM of paclitaxel (taxol) for 16 h. Cells were then fixed for TUNEL staining. For each experiment, apoptotic cells (stained green) were quantified among ~2000 total cells (stained blue), and the apoptotic index was computed by dividing the number of apoptotic cells by the number of total cells. ** indicates significance at P<0.01 level, relative to control nontargeting siRNA groups. (b) MCF-7 cells were transfected with control nontargeting siRNA or EIG121 siRNA for 48 h and then treated as indicated. Cells were ruptured, and EIG121, cleaved caspase 7, and β-actin were probed using specific antibodies. N, nontransfected cells (no siRNA); E, EIG121 siRNA; C, control nontargeting siRNA