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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: DNA Cell Biol. 2009 Nov;28(11):543–553. doi: 10.1089/dna.2009.0865

FIG. 6.

FIG. 6

Inhibition of PKCδ or STAT3 signaling attenuates the ET-1–mediated decrease in eNOS promoter activity in ovine FPAECs. FPAECs were cotransfected with pEGFP-N1-Prepro-ET-1 and a 1.6 kb eNOS promoter–luciferase reporter construct in the presence and absence of DN PKCδ or S727A STAT3. All cells were also transfected with a plasmid containing β-galactosidase as a transfection control. After 24 h, luciferase activity was measured. The ET-1–mediated decrease in eNOS promoter activity is attenuated by inhibiting either PKCδ or STAT3 activity (A). FPAECs were also transfected with 1.6 kb eNOS promoter–luciferase reporter construct in which the STAT3 binding element at −1024 was mutated. The overexpression of Prepro-ET-1 fails to inhibit eNOS promoter activity in cells transfected with mutant eNOS promoter (B). Values are mean ±SD. *p <0.05 versus control, †p <0.05 versus Prepro-ET-1 alone, n = 4.