Skip to main content
. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: DNA Cell Biol. 2009 Nov;28(11):543–553. doi: 10.1089/dna.2009.0865

FIG. 7.

FIG. 7

Inhibition of PKCδ or STAT3 signaling attenuates the ET-1–mediated decrease in NO signaling in ovine FPAECs. Ovine PAECs were cotransfected with pEGFP-N1-Prepro-ET-1 and either DN PKCδ or S727A STAT3. Cellular extracts (20 μg) were then subjected to Western blot analysis using a specific antibody raised against eNOS; a representative image is shown (A). The ET-1–mediated decrease in eNOS protein levels are significantly attenuated by DN PKCδ and S727A STAT3 (B). Similarly, DN PKCδ and S727A STAT3 reverses the ET-1–mediated inhibition of NO generation (C). Values are mean ± SD. *p <0.05 versus control, †p < 0.05 versus Prepro-ET-1 alone, n = 4.