Figure 4. Survival and weight changes in mice immunized with AL proteins and different adjuvants followed by intranasal VACV challenge.

Mice were immunized as described in Fig. 1, and three weeks following the second immunization, were challenged intranasally with 10⁶ pfu of VACV strain WR. The unchallenged (Unchall) mice were not immunized or challenged. The alum+CpG control group received no recombinant protein prior to challenge. Mice were weighed daily for two weeks and sacrificed if their weight fell below 70% of the initial value. The percent of survivors (A) and the percent of initial weight of surviving mice (B) are shown for each group. The data shown here represents two independent experiments and each group had 4-5 mice/group. Each data point is the average weight +/−SEM of mice in each group from the two challenge experiments