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. Author manuscript; available in PMC: 2010 Nov 26.
Published in final edited form as: J Neurosci. 2010 May 26;30(21):7249–7257. doi: 10.1523/JNEUROSCI.6341-09.2010

Figure 1. Chlorzoxazone decreases the irregularity of the intrinsic pacemaking of tottering Purkinje cells.

Figure 1

(A) Raw traces from extracellular recordings from wild-type (+/+) and tottering (tg/tg) Purkinje cells, with their interspike interval autocorrelograms below. Note the irregular firing of the tg/tg cell.

(B) Chlorzoxazone (CHZ) dose-dependently decreased the variation of the interspike interval in a tg/tg Purkinje cell whose spontaneous activity was extracellularly recorded.

(C) Average and individual values (circles) of the coefficient of variation of the interspike intervals in +/+ and tg/tg Purkinje cells. Note the higher values and the wider distribution of coefficient of variation in the mutant cells. (*) p<0.05, (**) p<0.01, (***) p<0.001 (One-way ANOVA, followed by Bonferroni correction), n=24 cells for tg/tg mice and n=19 cells for +/+ mice.

(D) Average and individual (circles) firing rates of the +/+ and tg/tg Purkinje cells shown in C, “n.s.” denotes not significant.

(E) CHZ increased the magnitude of the positive deflection of the spikes recorded extracellularly in tg/tg Purkinje cells. The expanded figure shows an averaged trace of the activity of 7 cells, in control and after adding CHZ 60 μM.

(F) Average magnitude of the positive deflection in control and after adding CHZ in increasing concentrations. (*) p<0.05 (One-way ANOVA, followed by Bonferroni correction).