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. Author manuscript; available in PMC: 2010 Nov 26.
Published in final edited form as: J Neurosci. 2010 May 26;30(21):7249–7257. doi: 10.1523/JNEUROSCI.6341-09.2010

Figure 2. Chlorzoxazone decreases the irregularity pacemaking through SK channels.

Figure 2

(A) The precision of pacemaking of a wild type cerebellar Purkinje cell was monitored by following coefficient of variation of its interspike intervals. Addition of low concentration of cadmium to partially block voltage-gated calcium channels made the firing of the cell irregular. 100 nM iberiotoxin was then added to block BK channels. Despite the blockade of BK channels, 30 μM CHZ restored the precision of pacemaking to control conditions.

The average coefficient of variation of interspike intervals (B) and firing rate (C) of 5 Purkinje cells following the experimental procedure described in panel (A). (*) p<0.05 (One-way ANOVA, followed by Bonferroni correction), “n.s.” denotes not significant.

(D) The pacemaking of a Purkinje cell was made irregular with addition of cadmium, and the selective BK channel activator NS1619 was bath applied at a concentration of μM. While NS1619 was ineffective in restoring the precision of pacemaking, subsequent addition of 30 μM CHZ returned the coefficient of variation of interspike intervals to pre-cadmium levels.

The average coefficient of variation of interspike intervals (E) and firing rate (F) of all the Purkinje cells examined following the experimental procedure described in panel (D). (*) p<0.05, “n.s.” denotes not significant.