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. Author manuscript; available in PMC: 2010 Jun 27.
Published in final edited form as: J Neurosci. 1997 Oct 1;17(19):7532–7540. doi: 10.1523/JNEUROSCI.17-19-07532.1997

Figure 1.

Figure 1

Diazepam was effective in controlling brief (10 min) seizures but lost efficacy after prolonged (45 min) seizures. Seizures were induced in 70–150 gm rats by intraperitoneal injection of LiCl at 3 mEq/kg followed 16–24 hr later by intraperitoneal injection of pilocarpine at 50 mg/kg. Behavioral seizures started within 1–5 min in all rats. Diazepam was administered 10 min (filled boxes, solid line; n = 14) or 45 min (filled circles, dashed line; n = 12) after pilocarpine injection. The percent of rats that stopped having seizures within 5 min of diazepam injection was plotted against the log of the diazepam dose. The data were fitted to a sigmoidal dose–response curve with the maximum fixed to 100% and the minimum to 0%. The ED50 values were derived from the equation that best fit the data.