WIN-2 evokes CGRP release from TG neurons: (a) Concentration–response curve for WIN-2-evoked CGRP release from TG neurons following 10 min exposure (n=6, all concentrations). (b) The calcium-dependence of WIN-2-evoked CGRP release was tested by the exclusion of calcium from the extracellular solution (n=6, ***P<0.001 vs baseline with calcium). (c) Both WIN-2 and the enantiomer, WIN-3, evoke CGRP release from TG neurons (n=6, **P<0.01, ***P<0.001 vs no drug control; +P<0.05, ++P<0.01 WIN-2 vs WIN-3 comparisons).