G-protein-coupled receptor dependence of WIN-2 inhibition of 50mM K+-evoked CGRP release: 10 μM WIN-2 inhibition of 50mM K+-evoked CGRP release was assessed in the presence of the CB1 antagonists, SR141716A (a) and AM251 (b), as well as the CB2 antagonist, AM630 (c), n=6, all experiments, P<0.001 vs K+alone). (d) The effect of Gi/o G protein uncoupling was tested by 18 h pretreatment with PTX (n=6, *P<0.05 vs K+alone). All data are presented as the sum of the agonist7antagonist pretreatment + the K+-evoked CGRP release treatment to account for the ability of WIN-2 to evoke CGRP release on its own.