Coronal sections of E16.5 embryos were examined for the expression of DA pathway genes. Immunofluorescence for tyrosine hydroxylase (TH, A,B), dopamine transporter (DAT, C,D) and the vesicular monoamine transporter 2 (VMAT2, E,F) were similar in Etv5−/− (KO) embryos and control (WT) mice. The expression pattern of the regulator of DA differentiation Nurr1 was also indistinguishable in Etv5−/− embryos and controls (G,H). SNc, substantia nigra, pars compacta; VTA, ventral tegmental area. Scale 80μm.