Etv5 expression was examined by ISH in sagittal sections at E14.5 (A), and in coronal sections at E16.5, P7 and P14 (C,E,G). Immunofluorescence for TH in adjacent sections was used to localize DA neurons in the ventral midbrain (B, D, F, H). Arrows in (A,B) indicate the location of differentiating SN/VTA neurons. Etv5 expression in the SNc was first detected at P7. 3V, third ventricle; SC, superior colliculus; SN, substantia nigra; thal, thalamus; VTA, ventral tegmental area. Scale 250μm.