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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Neuropsychopharmacology. 2010 Aug 25;35(12):2324–2338. doi: 10.1038/npp.2010.130

Figure 3. nAChR subunit mRNA expression patterns and radioligand binding indicates assembly of α5 subunit containing α4β2 nAChRs in the thalamo-cortico-thalamic circuitry of the mouse.

Figure 3

In-situ hybridization for β2, α4 and α5 nAChR subunit expression in comparable coronal sections taken from wild-type (WT), heterozygous (HET), β2 nAChR subunit knock out (KO) and corticothalamic transgenic (Tr(CT)) mice. HET, KO and Tr(CT) mice exhibit alterations in β2 mRNA expression with no significant changes in the α4 or α5 subunits. A85380 and epibatidine binding on comparable coronal sections from these animals also show differences in the numbers of assembled nAChRs in these mice. Epibatidine binding is eliminated in the presence of cytisine (Epi + cytisine) and corresponds to the binding of A-85380 in these brain regions. Representative sections at a level of approximately −1.6 mm Bregma are shown.