Table 1.
Filament | Lp (μm)b | κ (pN μm2)b | I (nm4)b | Eapp (pN nm-2)b | C (pN μm2 rad-1)c |
---|---|---|---|---|---|
Actin | 9.8 (± 0.14) | 40 (± 0.49)×10-3 | 120 | 330 | 2.30 (± 1.00) ×10-6 |
Cofilactin | 2.2 (± 0.026) | 9.1 (± 0.11)× 10-3 | 240 | 38 | 0.13 (± 0.06) ×10-6 |
Conditions: 20 mM imidazole (pH 6.6), 25°C, 50 mM KCl, 0.2 mM ATP, 2 mM MgCl2, 2 mM DTT, 1 mM NaN3
The values of the filament persistence length (Lp) flexural stiffness (κ), shape-dependent geometric moment of inertia (I) and apparent elastic Young's modulus (Eapp) are taken from McCullough et al. (2008)
The values of the filament torsional stiffness (C) are taken from Prochniewicz et al. (2005)