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. Author manuscript; available in PMC: 2010 Aug 11.
Published in final edited form as: Oncotarget. 2010 Jul 1;1(3):228–232. doi: 10.18632/oncotarget.130

Figure 2. TSC2 knockdown in Rb mutant cancer cells induces an increase in ROS.

Figure 2

roGFP1 is a redox-sensitive variant of EGFP which exhibits a decrease in florescence intensity in an oxidative state. Du145 cells containing either EGFP or roGFP1 were treated as shown. A-D) The fluorescence intensity of EGFP did not vary with redox status. E-H) The intensity of the florescent signal of roGFP decreased when cells were in an oxidative state (H2O2, G) as compared to control (E) but remained the same when treated with NAC, an inhibitor of ROS (H). Knocking down TSC2 (shTSC2) resulted in an oxidative state in the cells (compare E and F). For experimental details see reference [1].