Figure 6. Kisspeptin evoked current is attenuated by Li⁺ and KB-R 7943.

The current traces were evoked by voltage ramps from −130 to +10 mV at a holding potential of −60mV. TTX (0.5 µM), AP5 (50 µM), CNQX (10 µM), and Cd²⁺ (200 µM) were added in the bath solution. A, Recordings performed with a normal ACSF bath perfusion and a KMeSO4 pipette solution. Top, Traces showing the current responses to the voltage ramp in POMC cells before (Cont), and during kisspeptin (Kiss,1 µM). Bottom, the Kiss-induced current (Subtraction of Cont from Kiss). B, Cont and Kiss (Top) and Kiss-induced current (Bottom) recorded with BaCl₂ 0.3 mM in the bath perfusion and a CsMeSO4 pipette solution. C, Kiss-induced current recorded using a CsMeSO4 pipette solution in modified ACSF with Na⁺ replaced by Li⁺. D Kiss-induced current recorded using a CsMeSO4 pipette solution after pretreated with NCX blocker KB-R 7943 (60 µM). E, Bar graph showing the amplitude of Kiss-induced current at −125 mV under different recording conditions. *p<0.05, **p<0.01 vs normal ACSF, group t-test.