Skip to main content
. Author manuscript; available in PMC: 2010 Aug 16.
Published in final edited form as: Cell Mol Life Sci. 2007 Nov;64(22):2933–2944. doi: 10.1007/s00018-007-7206-8

Figure 2.

Figure 2

Designer molecular scissors for genome surgery. A schematic representation of a pair of 4-finger ZFNs (zinc finger nucleases) bound to their cognate sites in the IL2Rγ gene [4]. Since a pair of ZFNs requires two copies of the 12-bp recognition sites in a tail-to-tail orientation, they effectively have a 24-bp recognition site, which is long enough to specify a unique genomic address in human cells. Since the ZFN-binding sites in the IL2Rγ gene are not identical, both ZFNs that bind these sites need to be introduced into the cell to induce a targeted genomic DSB. The ZFN sites in the IL2Rγ gene are separated by 5 bp [4].