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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Cell Mol Med. 2011 Apr;15(4):957–969. doi: 10.1111/j.1582-4934.2010.01063.x

Figure 2.

Figure 2

Nuclear translocation of Cav-1 in IGFBP-5-expressing fibroblasts. A: 1 × 106 human lung fibroblasts were cultured on 100mm dishes and untreated or infected with cAd or Ad5 at an MOI of 50. After 24h, cytoplasmic and nuclear extracts were prepared and expression of Cav-1 was examined by western blot. Cytoplasmic extracts from 5 × 104 cell-equivalent or nuclear extracts from 1 × 105 cell-equivalent was applied to each lane. Alpha-tubulin is shown as a loading control for cytoplasmic extracts, and histone for nuclear extracts. Left panel; Non-treated cells (NT). Right panel; cAd- or Ad5-infected cells. B: Expression of IGFBP-5 and Cav-1 was examined by immunofluorescence. Human lung fibroblasts were cultured on cover slips coated with type I collagen. After infection using cAd (A–C) or Ad5 (D–G) for 48h, cells were stained for IGFBP-5 (green) and Cav-1 (red). Hoechst (blue) was used to identify nuclei. Magnification of images ranged from 800x to 1000x on a confocal microscope. Scale bars = 20 μm.