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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Cell Mol Med. 2011 Apr;15(4):957–969. doi: 10.1111/j.1582-4934.2010.01063.x

Figure 4.

Figure 4

IGFBP-5 (A–C), IGFBP-3 (D), and Egr-1 (E) levels in cytoplasmic and nuclear extracts from WT and Cav-1−/− fibroblasts. Cytoplasmic and nuclear fractions were extracted from WT and Cav-1−/− fibroblasts that were untreated or infected with the indicated adenoviral constructs for 48 h. Cytoplasmic and nuclear extracts were subjected to immunoblotting for the detection of IGFBP-5, IGFBP-3, and Egr-1. Alpha-tubulin is shown as a loading control for cytoplasmic extracts, and histone for nuclear extracts. F: Graphical summary of data is shown in panels B–E. The signal intensity of each protein in nuclear extracts was normalized to histone, and the ratio of the intensity in Cav-1−/− to WT was calculated. Differences in levels between WT and Cav-1−/− cells were compared using the unpaired t-test. Horizontal bars indicate mean values of 2 independent experiments. ** P < 0.01.