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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Sci China Chem. 2010 Jan 1;53(1):52–60. doi: 10.1007/s11426-010-0011-5

Figure 5.

Figure 5

Probing metal binding properties of predicted EF-hand Ca2+ binding motifs from Shr of Streptococcus pyrogenes (CD2.Shr.EF) and from the nsP1 of Sindbis virus (CD2.Sin.EF). (a) Modeled structure of the engineered protein with the insertion of a 29-residue helix-loop-helix EF-hand motif. (b) Far ultra-violet circular dichroism spectra of CD2 wild type and the engineered proteins. (c) and (d) Tb3+-binding curves of the engineered proteins CD2.Shr.EF and CD2.Sin.EF. The titration curve is fitted for a 1:1 binding stoichiometry. Figure 5 is the data from our study.