Skip to main content
. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: J Neurosci. 2010 Aug 18;30(33):11073–11085. doi: 10.1523/JNEUROSCI.0983-10.2010

Figure 3. S-SCAM links to β-catenin and neuroligin in CG neurons.

Figure 3

β-cat (lane 1) and NL (lane 3) co-immunoprecipitated with S-SCAM from CG lysates. In contrast, IQGAP (lane 8) did not. S-SCAM itself precipitated with the S-SCAM antibody (lane 5), but not with the hemagglutinin (HA) antibody, as a negative control (lane 6). CG homogenates were immunoprecipitated (IP) with S-SCAM or HA antibody. The precipitate and 1–2% of total input were separated by SDS-PAGE and immunoblotted (IB) with β-cat antibody (lanes 1 and 2), NL antibody (lanes 3 and 4), S-SCAM antibody (lanes 5–7), or IQGAP antibody (lanes 8 and 9). β-cat ~92 kDa; NL ~110 kDa; S-SCAM isoforms: α (full length), β and γ, 180, 160, and 105 kDa; IQGAP ~190 kDa. n = 3 separate experiments.