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. Author manuscript; available in PMC: 2010 Sep 17.
Published in final edited form as: Genes Cancer. 2010 May 1;1(5):506–515. doi: 10.1177/1947601910376192

Figure 2.

Figure 2

Zyxin is phosphorylated upon UV irradiation. (a) Western immunoblot reveals that zyxin migrates more slowly in SDS-PAGE when isolated from UV-C–irradiated cells (lanes 1–2), and this is reversed if the sample is treated with Calf Intestinal Phosphatase (CIP) prior to electrophoresis (lanes 3–4). Zyxin with Ser336 mutated to alanine to block phosphorylation does not shift to a slower mobility form upon UV-C exposure (lanes 5–6). (b) Diagram of zyxin residues that were shown to be phosphorylated by mass spectroscopy analysis of phosphopeptides generated from purified zyxin. (c) Site directed mutagenesis to convert the phosphorylatable Serine residues to alanine did not adversely impact zyxin’s ability to localize at focal adhesions (arrowheads).