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. Author manuscript; available in PMC: 2010 Sep 17.
Published in final edited form as: Genes Cancer. 2010 May 1;1(5):506–515. doi: 10.1177/1947601910376192

Figure 3.

Figure 3

Identification of an apoptotic regulator as a zyxin-binding partner. (a) Schematic of the GZT fusion protein: GFP, green fluorescent protein; α-A, α-actinin binding site; ActA proline rich repeats; NES, nuclear export sequences; L1-2-3, LIM domains; ProteinA tag. (b) Immunoblot analysis of GZT protein expression using anti-zyxin serum, in Zyx−/− and GZT cells. (c) Subcellular localization of the GZT protein mirrors that of wild-type zyxin. (d) Schematic representation of the strategy used to isolate zyxin protein complexes. (e) Silver staining of affinity-purified products from Zyx−/− and GZT cells after SDS-PAGE. Bands specific to GZT cells were identified by mass spectrometric analysis to be CARP-1 and Mena.