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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: J Neurosci. 2010 Aug 25;30(34):11369–11378. doi: 10.1523/JNEUROSCI.0529-10.2010

Figure 5. DfhIR larvae accumulate excess synatobrevin-containing vesicles in their synaptic NMJs at 3rd instar compared to controls.

Figure 5

(A) Synaptic (synaptobrevin-containing) vesicles in motor neurons were visualized by D42-syb-GFP (green) and NMJs were counter-stained with anti-HRP antibody (red, overlap with green shown as yellow), late 3rd instar is shown here. Synaptic vesicle densities were determined as the ratio of the number of syb-GFP pixels to the number of HRP pixels at 2nd, early 3rd and late 3rd instar (B). Late 3rd instar DfhIR larvae show increased density of synaptic vesicles in their NMJs relative to wild type. All error bars represent the sd and significant differences between DfhIR and wild type NMJs are indicated (asterisk p<0.05, n=40 for all experiments). Scale bar, 2.5 µm.