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. Author manuscript; available in PMC: 2010 Nov 26.
Published in final edited form as: Oncotarget. 2010 Sep 1;1(5):329–338. doi: 10.18632/oncotarget.165

Figure 3. CD277 inhibits cFLIP in activated human T cells.

Figure 3

(A) Resting human T cells or T cells activated for 24 hours with anti-CD3/anti-CD28 beads were incubated with a Myc-tagged irrelevant protein or a with Myc-tagged extracellular form of CD277. Protein binding to the cell surface was analyzed by FACS using a secondary anti-Myc antibody. Data are representative of three independent experiments with similar results. iProt, irrelevant protein. eCD277, extracellular region of CD277. (B) Human T cells were cocultured for 24 hours with K32 cells ectopically expressing or not CD277 and whole cell lysates were used to analyze c-Flip expression by Western blot. Data are representative of two independent experiments with similar results. (C) Resting human T cells were activated for 24 hours with K32 cells ectopically expressing (solid) or not (open) CD277 and intracellular expression of Annexin V was determined.