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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Stem Cells Dev. 2010 Sep 14;20(3):475–484. doi: 10.1089/scd.2010.0219

FIG. 2.

FIG. 2

Oct4 expression is detected in human embryonic stem cells (hESCs) by FRET using confocal microscopy. Typical confocal micrographs of aggregated hESCs in suspension transfected with 6FAM (donor)- and 5ROX (acceptor)-labeled, Oct4-specific molecular beacons separately and in combination are shown. The same field is shown across each row, with each panel produced using the indicated excitation wavelength/detection filter combination. Individual beacons fluoresce at appropriate wavelengths (6FAM, upper left; 5ROX, middle middle), with absence of overlapping emissions (6FAM, middle left; 5ROX, upper middle). Only cells transfected with both beacons fluoresce at the acceptor’s wavelength (610 nm) when excited within the donor’s absorption spectrum (488 nm) (6FAM+5ROX, right lower). Scale bar: 100 μm. Color images available online at www.liebertonline.com/scd.