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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Stem Cells Dev. 2010 Sep 14;20(3):475–484. doi: 10.1089/scd.2010.0219

FIG. 6.

FIG. 6

FRET-based FACS of Oct4-expressing hESCs achieves enrichment of pluripotency. (A) Typical teratomas produced from 105 FRET-positive hESCs (FRET), unsorted hESCs, or mouse embryonic fibroblasts (MEFs) are shown. (B) The ratio of teratoma+kidney weight:contralateral kidney weight is significantly higher for FRET-positive hESCs than for unsorted hESCs, suggesting enrichment for pluripotent hESCs. As expected, MEFs did not produce teratomas. Weight axis (left) applies to columns for teratoma and kidney weights; teratoma/kidney ratio axis (right) applies to columns for teratoma/kidney ratios. Data shown represent mean ± standard error of the mean (SEM) (n = 2). Color images available online at www.liebertonline.com/scd.