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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: J Neurosci. 2010 Sep 15;30(37):12446–12454. doi: 10.1523/JNEUROSCI.1667-10.2010

Figure 4.

Figure 4

β1 adrenoceptors promote sympathetic neurite outgrowth. Superior cervical sympathetic ganglion neurons cultured without treatment extend many neurites (A). Addition of propranolol at a concentration of 10−10 M appeared to increase neurite outgrowth (B) and this was confirmed by quantitative analysis of peripherin-immunoreactive neurite area (C; n=12 per condition). Cultures treated in the presence of 10−8M metoprolol (D) showed increased neurite outgrowth similar to propranolol, and this was confirmed by quantitative analysis (E; n=12). Treatment with the β2 antagonist ICI 118,551 had no effect on neurite outgrowth (F; n=3). Counts of neuronal somas per unit area showed no differences between control cultures and those treated with propranolol (G) or metoprolol (H). Scale bar in D = 50μm for all micrographs. Results are presented as the mean ± s.e.m. *P<0.05 compared to control.