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. Author manuscript; available in PMC: 2011 Apr 27.
Published in final edited form as: J Neurosci. 2010 Oct 27;30(43):14458–14469. doi: 10.1523/JNEUROSCI.2366-10.2010

Figure 2.

Figure 2

dPER(ΔC4) interacts with TIM but not with dCLK in S2 cells. S2 cells were transiently co-transfected with different versions of pAct-dper in combination with pMT-dClk-V5 (A), or pAct-tim-3HA (B). Expression of recombinant dCLK was induced 24 hr after transfection by adding 500 μM CuSO4 to the medium. Cells were harvested 24 hr after induction (A) or 36 hr post-transfection (B), and protein extracts were either directly analyzed by immunoblotting (Input) or following immunoprecipitation (IP). (A) IP was performed in the presence of anti-dPER antibodies (P) or a non-specific antibody (HA), and immune complexes analyzed for dCLK. (B) IP was performed in the presence of anti-TIM antibodies and immune complexes analyzed for dPER.