Table 1.
Prevalence of C-terminal Domain Mutations in RTI Treatment-experienced Patients.
| C-terminal domain mutation | No. of sequences containing a C-terminal domain mutation a | No. of sequences containing a C-terminal domain mutation and no RTIb mutations | No. of sequences containing both a C-terminal domain mutation and ≥1 RTI mutation | Probability of having a C-terminal domain mutation with ≥1 RTI mutation c |
|---|---|---|---|---|
| Connection Subdomain | ||||
| E312Q | 79/6035 (1.3%) | 36/3397 (1.1%) | 43/2638 (1.6%) | *P = 0.0350 |
| Y318F | 48/5983 (0.8%) | 0/3366 (0%) | 48/2617 (1.8%) | *P < 0.000001 |
| G333D | 47/5086 (0.9%) | 21/2864 (0.7%) | 26/2222 (1.2%) | P =0.0717 |
| G333E | 446/5086 (8.8%) | 221/2864 (7.7%) | 225/2222 (10.1%) | *P = 0.0016 |
| G335C | 30/4905 (0.9%) | 14/2711 (0.5%) | 16/2194 (0.7%) | P = 0.2211 |
| G335D | 79/4905 (1.6%) | 37/2711 (1.4%) | 42/2194 (1.9%) | P = 0.0802 |
| N348I | 180/3189 (5.6%) | 5/1213 (0.4%) | 175/1976 (8.9%) | *P < 0.000001 |
| A360V | 128/3147 (4.1%) | 17/1203 (1.4%) | 111/1944 (5.7%) | *P < 0.000001 |
| V365I | 169/3140 (5.4%) | 36/1202 (3.0%) | 133/1938 (6.9%) | *P = 0.000001 |
| T369I | 19/3115 (0.6%) | 1/1195 (0.08%) | 18/1920 (0.9%) | *P = 0.0013 |
| A371V | 518/3112 (16.6%) | 47/1194 (3.9%) | 471/1918 (24.6%) | *P < 0.000001 |
| A376S | 320/3111 (10.3%) | 87/1194 (7.2%) | 233/1917 (12.2%) | *P = 0.000006 |
| E399D | 475/2968 (16%) | 178/1072 (16.6%) | 297/1896 (15.7%) | P = 0.7656 |
| A400T | 455/1616 (28.2%) | 205/628 (32.6%) | 250/988 (25.3%) | P = 1.00 |
| RNase H Domain | ||||
| Q509L | 2/507 (0.4%) | 2/304 (0.7%) | 0/203 (0%) | P = 1.00 |
Data from the Stanford HIV Drug Resistance Database, Detailed RT Mutation Profile Program, as of April 2010.
Major and minor RTI mutations were those defined by the Stanford database. Major NRTI mutations (http://hivdb.stanford.edu/pages/documentPage/NRTI_mutationClassification.html) included 41L, 65R/N, 67N, deletion D67, insertion at T69, 69D, 70R/E/G, 74I/V, 75T/A/M, 115F, 151M/L, 184V/I, 210W, and 215Y/F. Minor NRTI mutations included 41 not L, 44D/A, 62V, 67 not deletion or N, 69 not insertion or D, 70 not R/E/G, 74 not I/V, 75 not T/A/M, 77L, 115 not F, 116Y, 118I, 151 not M/L, 184 not V/I, 210 not W, 215 not Y/F, 219 Q/E/N/R/W, 333D/E, and 348I. Major NNRTI mutations (http://hivdb.stanford.edu/pages/documentPage/NNRTI_mutationClassification.html) included 100I, 101E/P, 103N/S/T/H, 106A/M, 179F, 181C/I/V, 188C/H/L, 190A/S/E/Q/T/C/V, 230L, and 236L. Minor NNRTI mutations included 90I, 98G, 100 not I, 101 Q/H/N, 103 not N/S/T/H, 106 not A/M/I/L, 108I, 138K, 179D/E, 181 not C/I/V, 188 not C/H/L, 190 not A/S/E/Q/T/C/V, 225H, 227C/L, 234I, 236 not L, 238N/T, 318F and 348I. The word “not” refers to all mutations at that position except the following mutation(s).
Two proportions statistics were performed by comparing the number of C-terminal domain mutations with at least one RTI mutation (for example, 43 for E312Q) to the total number of sequences containing at least one RTI mutation (2638 of 6035), against the number of C-terminal domain mutations without an RTI mutation (for example, 36 for E312Q) to the total number of sequences without an RTI mutation (3397 of 6035).