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Perturbation of the redox state in LNCaP and PC3 cells upon PKC suppression. (A) Following treated with HMG, the cells were stained with DCF, and analyzed for ROS levels using a flow cytometer. Error bar represents SD over 5 independent experiments (n=5, p<0.05). (b and c) The expression of HO-1 (B) or GADD153 and BiP (c) in the cells, after PKC suppression, was tested by immunoblotting. Equal loading total proteins were verified by actin expression.
