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. Author manuscript; available in PMC: 2011 May 11.
Published in final edited form as: Neuropsychopharmacology. 2010 Dec 8;36(4):772–781. doi: 10.1038/npp.2010.211

Figure 4.

Figure 4

Virgin AC3−/− female mice fail to detect odorants and pup urine. (a) Odorant habituation data for AC3+/+ and AC3−/− virgin female AC3−/− mice are reported. Cotton swabs were laced with 50 µl of citralva (10 µM), 2-heptanone (50 µM), male urine (20-fold diluted), or pup urine (20-fold diluted). There were significant differences in the ability of AC3+/+ (n=10) and AC3−/− (n=10) mice to detect citralva (p< 0.001), 2-heptanone (p< 0.01), male urine (p< 0.01), and pup urine (p< 0.001). Data are represented as means ± SEM. (b and c) Virgin female AC3+/+ mice but not virgin AC3−/− females detected anesthesized pups. Non-visual pup detection was assayed as described in Materials and Methods using anesthesized pups. (b) During context habituation, AC3+/+ (n=8) and AC3−/− (n=8) female mice sniffed each side chamber equally. However, during testing (c) AC3+/+ female mice, but not AC3−/− females, showed a strong preference for the side chamber containing the anesthesized pup (p< 0.001). Data are presented as means ± SEM. *, p < 0.01; **, p < 0.001.