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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Neurosci. 2010 Dec 1;30(48):16170–16179. doi: 10.1523/JNEUROSCI.2323-10.2010

Figure 5. Effects of the N-terminus and C-Linker/AC region of mSlo1 in dSlo1 background on the β2ND modulation of Ca2+ sensitivity.

Figure 5

A, Macroscopic currents of d[mS0], d[mLinker+mAC] and d[mS0+mLinker+mRCK1c] with and without β2ND at ~100 μM [Ca2+]i. Voltage pulses are from −200 to 200 mV except for d[mS0+mLinker+mRCK1c]+β2ND, which are from −200 to 100 mV, with 10 mV increment. The repolarizing potential is −50 mV. The dashed line represents the zero current line for each current trace. B, G-V relations of chimeras with and without β2ND at low (0 or ~2 μM) and ~100 μM [Ca2+]i as compared with that of dSlo1 (blue). C, V1/2 vs. [Ca2+]i plots of chimeras compared with that of mSlo1 and dSlo1 with and without β2ND. The number of patches for each data are: for the following ~(μM) [Ca2+]i, 0, 1, 2, 5, 10, 30, 100, d[mS0]: 0, 0, 3, 10, 6, 4, 20; d[mS0]+β2ND: 0, 0, 0, 3, 2, 3, 16; d[mLinker+mAC]: 13, 5, 4, 4, 4, 4, 7; d[mLinker+mAC]+β2ND: 23, 5, 3, 3, 5, 5, 13; d[mS0+mLinker+mRCK1c]: 11, 2, 4, 7, 6, 16; d[mS0+mLinker+mRCK1c]+β2ND: 15, 5, 5, 5, 6, 14.