Table 1.

Intranigral administration of poly(I:C) increases the level proinflammatory cytokines and chemokines.

Cytokine Chemokine	Substantia Nigra protein level 12 days post poly (IC) (pg/mg)
	Vehicle	Poly (IC)
IL-1α	7.993 ± 1.558	12.32 ± 1.208 *
IL-1β	5.564 ± 0.5115	6.183 ± 0.3726
IL-2	4.926 ± 0.7362	4.772 ± 0.3633
IL-4	5.910 ± 1.034	5.279 ± 0.4931
IL-6	55.19 ± 3.111	51.55 ± 2.167
IL-10	ND	ND
IFN-γ	6.618 ± 0.4500	8.275 ± 1.364
TNF-α	23.05 ± 3.670	25.25 ± 6.779
MCP-1	6.501 ± 0.9393	9.541 ± 0.9878 *
MCD	1.959 ± 0.1989	2.195 ± 0.1701
Fractalkine	67.93 ± 3.127	69.00 ± 2.411
MIP-1α	10.12 ± 1.698	11.04 ± 1.224
MIP-2α	4.860 ± 0.6166	5.827 ± 0.5258
MIP-3α	96.18 ± 7.500	105.4 ± 7.355
RANTES	63.96 ± 3.495	96.27 ± 5.275 **
TGF-β1	258.1 ± 24.64	246.1 ± 15.16

Cytokine Chemokine	Striatum protein level 12 days post poly (IC) (pg/mg)
	Vehicle	Poly (IC)
IL-1α	13.29 ± 2.285	11.98 ± 1.279
IL-1β	4.728 ± 0.4303	6.937 ± 0.5908 **
IL-2	5.124 ± 0.4348	5.790 ± 0.4551
IL-4	6.394 ± 0.9461	5.232 ± 0.4566
IL-6	95.59 ± 6.001	114.5 ± 5.941*
IL-10	ND	ND
IFN-γ	9.206 ± 2.411	9.822 ± 2.148
TNF-α	24.31 ± 4.697	39.42 ± 3.461 *
MCP-1	2.174 ± 0.3089	3.365 ± 0.3347 *
MCD	1.959 ± 0.1989	2.195 ± 0.1701
Fractalkine	142.8 ± 4.006	136.3 ± 3.928
MIP-1α	5.000 ± 0.5016	5.479 ± 0.5343
MIP-2α	4.228 ± 0.4588	4.770 ± 0.3614
MIP-3α	81.08 ± 4.540	86.95 ± 6.358
RANTES	73.06 ± 6.524	76.51 ± 7.677
TGF-β1	161.3 ± 10.06	202.4 ± 5.722 **

Cytokine and chemokine levels were analysed in the SN and striatum at 12 days after poly (I:C) injection using multiplex ELISA. Poly(I:C) injection caused increased levels of IL-1α, MCP1 and RANTES within the SN and IL-1β, IL-6, TNF-α, MCP-1 and TGF-β in the striatum. Data are shown as mean ± SEM (vehicle, n=9; poly(I:C), n=10;

^*p<0.05

^**p<0.001 unpaired t-test).