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. Author manuscript; available in PMC: 2011 Jun 8.
Published in final edited form as: J Neurosci. 2010 Dec 8;30(49):16485–16497. doi: 10.1523/JNEUROSCI.3127-10.2010

Figure 4. EHD1 localizes prominently to EEA1-positive early endosomes in neurons.

Figure 4

(A) Neurons were transfected with FLAG-EHD1 (red) and stained for endogenous EEA1 (cyan) (co-localization appears white; arrowheads). Single channels as well as overlaid channels are shown for the somatodendritic region and a part of dendrite. (A’) Representative intensity line scans of FLAG-EHD1 and EEA1 are shown for a part of dendrite. Overlapping peaks for FLAG-EHD1 (red) and EEA1 (cyan) are visible. (B) Neurons were transfected with FLAG-EHD1 and stained for endogenous NEEP21. (B’) Representative intensity line scans of FLAG-EHD1 and NEEP21 are shown for a piece of dendrite. (C) Neurons were co-transfected with FLAG-EHD1 and rab11-GFP. (C’) Representative intensity line scans of FLAG-EHD1 and rab11-GFP are shown for a piece of dendrite.