Fig 1.
5HT3aR-BACEGFP cells of the somatosensory cortex are 5HT3aR-expressing GABAergic interneurons. A-B, Double in situ hybridization for 5HT3aR and EGFP/GAD67. A, Double in situ for 5HT3aR and EGFP show complete overlap in the adult somatosensory cortex. B, All 5HT3aR positive cells are also GAD67 positive. C-D, immunohistochemistry for GFP and Satb2/Olig2. C, No overlap with pyramidal cell markers as Satb2 confirms the interneuron identity. D, No EGFP-positive cells express oligodendrocyte marker Olig2. Scale bars = 100 μm. dISH, double in situ hybridization; IHC, immunohistochemistry.