Expression of Atp2b4 (B), Car6 (D), Cftr (E) and, Slc4a2 (F) all showed a significant decrease in mRNA expression in the absence of MMP20. Expression of Atp2b1 (A) and Car2 (C) were not significantly altered in the Mmp20 null mouse. Although a trend existed, the data for Slc4a4 (G) was not significant due to variability within the data sets. Data are presented as mean + SEM and represent measurements of six individual mice with duplicate measurements for each mouse (n=6). Results are presented as relative gene expression normalized to the geometric mean of Eef1α1, Gapdh, β-actin and Casc3 mRNA expression. Statistical analysis was determined by T-test (*, p<0.05, ** p <0.01, *** p<0.001).