TABLE 1. Gene specific primers for quantitative PCR.

Primers were designed by analysis of annealing sites by DNAStar Lasergene software and designed to flank intron-exon boundaries or were taken from PrimerBank (Spandidos et al., 2008; Wang and Seed, 2003). n/a not applicable. °C = annealing temperature.

	GenBank ID #	5′ Primer	3′ Primer	PrimerBank ID	°C
Klk4	NM_019928	CTGGCAGCCGGATGTTAGAGG	AGGAGTGGGGCATTGGGTAGC	n/a	64
Odam	NM_027128	GCTTTTGACAGCTTTGTAGGC	AAGCAGGCTTCCTTCTACTGG	n/a	60
Atp2b1	NM_026482	GCCATCTTCTGCACCATTG	CCGCCAAACTGCACAATTA	n/a	64
Atp2b4	NM_213616	AGATGTCGGGTTTGCTATGG	ATGATGTCTGACGCCTCCTT	n/a	63
Car2	NM_009801	ACTGGAACACCAAATATGGGGA	GCAAGGGTCGAAGTTAGCAAAG	31981657a3	63
Car6	NM_009802	CTAACAACGGACACACAGTATCG	GCCTTTGAGATGAACTCAGTGC	28461317a2	63
Cftr	NM_021050	CCGGTGACAACATGGAACACATAC	CCAGTACGCACCAAATCAGCACTA	n/a	63
Slc4a2	NM_009207	AACTTCGTACCTTAGGTGTGGA	GGCGGTGGTATTCAAAGTCTT	6678021a2	63
Slc4a4	NM_018760	GAAGGTCACCACACGATCTACA	TCCACATCAGATTTGTCGGAGT	9055346a1	64
Ef1α1	NM_010106	ATTCCGGCAAGTCCACCACAA	CATCTCAGCAGCCTCCTTCTCAAAC	n/a	62
Actb	NM_007393	TGACGGCCAGGTCATCACTATT	ACCCAAGAAGGAAGGCTGGAAA	n/a	65
Casc3	NM_138660	GGATCGGAAAAACCCAGCCTACAT	TGTTCCCAGAGACCCTCATCTT	n/a	65
Gapdh	NM_008084	GCAAAGTGGAGATTGTTGCCAT	CCTTGACTGTGCCGTTGAATTT	n/a	64