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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Stem Cells Dev. 2010 Nov 9;20(6):1021–1029. doi: 10.1089/scd.2010.0198

Figure 2. Expression of CXCR4 and CXCR7 by mNPCs.

Figure 2

A) Mouse NPCs are multipotent. Triple staining is shown for astrocytes (GFAP, blue), neurons (Beta3 tubulin, red), and oligodendrocytes (O4, green). B) Floating neural spheres prior to trituation to single cell suspension. C) After trituation, the single cell suspension was plated and expanded as neural progenitor cells (NPCs). D) RT-PCR for mRNAs of CXCR4, CXCR7, and GAPDH. Bottom panels show no reverse transcriptase controls for each primer set. E-G) Immunocytochemistry for CXCR4. H-J) Isotype controls for CXCR4 (rabbit isotype). K-M) Immunocytochemistry for CXCR7. N-P) Isotype controls for CXCR7 (mouse isotype).