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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Stem Cells Dev. 2010 Nov 9;20(6):1021–1029. doi: 10.1089/scd.2010.0198

Figure 3. Knockdown of SDF-1 secretion in CD133dMSCs by lentiviral shRNA.

Figure 3

CD133dMSCs were transduced with puromycin-selectable lentivectors expressing GFP (transduction control), scrambled (non-specific) shRNA or sequence-specific shRNA (against SDF-1). A) FACS of control cells (no label) and those transduced with GFP lentivector and selected by puromycin to determine cell purity after selection regime. B) ELISA for SDF-1 showed a 94% and 89% reduction in level of secreted SDF-1 from CD133dMSCs after lentiviral transduction of 2 different short hairpin RNA sequences for the SDF-1 transcript compared with a scrambled vector. SDF-1 secretion levels were not different from cells that received the scrambled vector and cells that were not transduced (no vector control). All measurements were performed in triplicate. C) Phase contrast microscopy images of CD133dMSCs that received lentivectors and no vector control cells (magnification 10x).