A, B) mNPC survival was significantly reduced after 48 hrs of hypoxic culture when treated with shSDF-1 CdM compared with shScram CdM. C) Addition of recombinant SDF-1 to alpha MEM containing 20ug/ml heparin increased mNPC survival in a dose-response manner: 0.5 ng/ml SDF-1: 18.3 ± 5.9% increase, P = 0.022; 1 ng/ml SDF-1: 26.7 ± 8.8% increase, P = 0.016. N.S., not significant; *P ≤ 0.05; **P ≤ 0.01; †P ≤ 0.01. GM, NSC growth medium. SFM, serum-free alpha MEM (base medium).