Skip to main content
. Author manuscript; available in PMC: 2011 Apr 12.
Published in final edited form as: Cell Cycle. 2011 Jan 15;10(2):323–336. doi: 10.4161/cc.10.2.14552

Fig 1. In vivo co-precipitation of Cdc18 and Swi6.

Fig 1

Cell extracts were prepared from transformants expressing pREP4X cdc18 3HA and pREP81X FlagSwi6. In vivo binding studies were performed by immunoprecipitating cells extracts on FLAG M2-agarose conjugate beads in the absence (F1, B1) or presence (F2, B2) of FLAG peptid or by incubating the extracts on Sepharose beads (F3, B3). Immunoblotting was done with F7 anti-HA antibodies for cdc18 and anti-FLAG or polyclonal antibody for Swi6. I input, F flow through; B beads.