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. Author manuscript; available in PMC: 2011 Apr 12.
Published in final edited form as: Cell Cycle. 2011 Jan 15;10(2):323–336. doi: 10.4161/cc.10.2.14552

Fig 6. Timing of centromere replication in cdc18I-43A and Δswi6.

Fig 6

Wild-type (FY2318), Δswi6 (FY3812), cdc18-I43A (FY3813), and swi6-F324A (FY5048) cells were synchronized at G1 by nitrogen starvation and released into 10 mM of hydroxyurea and 100 μg/ml of BrdU. DNA replication was determined by BrdU ChIP. (A) Replication timing at euchromatin ars2004. (B) Centromeric replication timing in cdc18-I43A, Δswi6 or swi6-F324A. Schematic representation of the structure of the three centromeres and the location of the primers used for ChIP. The dh and dg primers recognize sequences in all three centromere. The primers for imr1 are cen1 specific. The primers for cnt are able to detect cen1 and 3. All experiments were repeated three times and representative data are shown.